Rapidly detect as little as 0. PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target. Prepare the rest of the standards , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. 50 pg to 2 µg. The total amount of DNA expected is in the range of 3–100 ng, with concentrations as low as 0. 995 mL TE for the . Rapidly detect as little as 0. We quantified the extract with Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents (Thermos Fisher Scientific, Waltham, MA, USA) to evaluate the amount of residual DNA. The QuantiFluor® dsDNA Dye offers greater sensitivity over absorbance at 260nm (NanoDrop®) low-concentration samples, and better or equal sensitivity performance when compared to PicoGreen®. For Research Use Only. 1X dsDNA assay (%) 100 17. Quant-iT Assay Kits Quant-iT DNA Assay Kit, High Sensitivity The Quant-iT High-Sensitivity DNA Assay Kit has a linear detection range of 0. quant it picogreen dsdna assay kit reagents. DNA was normalized to 2 concentrations, 100+/-20µg/mL and 20+/-5µg/mL. 05 to consider statistical significance. • Suitable for use in 96- and 384-well plate formats. Rapidly detect as little as 0. The assay is highly selective for double-stranded DNA (dsDNA) over RNA (Figure 1, page 7) and is accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. Average size fragment was determined using a LabChip GX (PerkinElmer) instrument. For duplicate-well droplet. Catalog Number. According to the obtained results, the assay presents a linear correlation in a wide range of 0. 500/525. no. Using 20x TE buffer make 1x solution. Average 86 stars, based on 1. dsDNA and PicoGreen analyzed using the Modulus and the Blue Fluorescence Optical Kit. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. 95) in the range of 10 pg till 100 ng for the salmon dsDNA as well as for the control dsDNA from the kit (λ dsDNA standard) (Fig. 21 DiB) [22]. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Calibrator MPO. Fluorometric dsDNA Assay. P11496). Picogreen assays are compatible with most microplate readers and fluorometers. Material Amount Concentration Storage Stability Quant-iT™ PicoGreen® dsDNA reagent (Component A) 1 mL in 1 vial (P7589) or in10 vials of 100 μL each (P11496) Solution in DMSO 2–6ºC Desiccate Protect from light • • • When stored as directed, product stable. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. PicoGreen dsDNA quantitation reagents are. 0 ml) of the aqueous working solution of Quant-iT™ PicoGreen dsDNA Reagent was added to each cell sample, which was incubated for 10 min at 37 °C in the dark. 9, 10 The Pico-Green assay is sensitive to 25 pg dsDNA/mL, which is 400-fold more sensitive than Hoechst 33258 dye. Fluorescence was measured at. 适用于（设备）. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM製品番号（カタログ番号）： P11495. 710 citations – Kit [P7589] Quant-iT™ PicoGreen™ dsDNA Assay Kit by Thermo Scientific. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. 2000 ng/mL of lamda dsDNA was serially diluted in 1XTE before the addition of the PicoGreen working solution. 50 ul PicoGreen Mix by swirling bottle. Rapidly detect as little as 0. Quant-iT PicoGreen dsDNA Assay Kit. , QuantIt PicoGreen dsDNA assay). Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 99 to $1999. Quant-iT PicoGreen dsDNA Assay Kit a. dsDNA • Quant-iT PicoGreen dsDNA Assay Kit (Cat. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. Quant-iT™ PicoGreen ® dsDNA Assay Kit. Application observe demonstrating so with Molecular Devices SpectraMax® microplate book and the Quant-iT PicoGreen assay, users can reliably size concentrations as low as 50 pg/mL of double-stranded DNA. PicoGreen dsDNA quantitation reagents are. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 0 fluorometer according to the manufacturer’s protocols; a. Absorbance-based measurements employ the DNA as it is and volumes of 100 µl, 50 µl, and 2 µl were placed into a 96 well, 384 well, or LVis plate, respectively. 5 – 1,000 ng/ mL DNA with a high dynamic range (Fig. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Natural cures with herbs are. 2. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. Quant-iT™ PicoGreen™ dsDNA Assay KitInvitrogen - Thermo FisherCatalog #P11496 Quant-iT™PicoGreen® dsDNA reagent is dissolved in dimethylsulfoxide (DMSO), which freezes below 19 °C. For example, if the assay requires 50 wells, add 600 L of PicoGreen to 14. Catalog number: P7581. This kit contains. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA, with a linear range from ~1 – 3,000 pg (depending on instrument capability). Quant-iT PicoGreen dsDNA Assay Kits and Reagents are designed to selectively detect dsDNA from a wide variety of sources, even in presence of ssDNA, RNA, and free. Figure 2. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. . Dilute from 20xTE stock (supplied in the PicoGreen kit). The assay is highly selective for RNA over double-stranded DNA, and provides a dynamic range of 5-100 ng of RNA. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 1 mL kit. PicoGreen® Assay Supplies Equipment: − NanoDrop 3300 Fluorospectrometer − 2uL pipettor (low retention nuclease free pipette tips) Materials:Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). PicoGreen dsDNA quantitation reagents are. Dynamic range and sensitivity of the Quant-iTTM PicoGreen® dsDNA assay. DNA. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). Q33130 Introduction The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation. Regulations & Guidance; Manuals 0 0. • Specific for dsDNA in the presence of RNA. Price from $9. The final pooled sample was quantified using a Quant-IT PicoGreen dsDNA assay (Thermo Fisher Scientific) and the resulting fragment size distribution was assessed using a Bioanalyzer (Agilent. Libraries were sequenced on the Illumina NovaSeq. The Quant-iTTM dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. This protocol is a simplified and c. Genomic DNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. were treated with standard-of-care chemotherapy in vitro followed by cfDNA quantification via a PicoGreen dsDNA assay. • Specific for dsDNA in the presence of RNA. 3ng/mL，DNAThe fragment size of the amplicon was assessed using agarose gel electrophoresis and quantified by Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). Effect of the buffer dilution on the DNA measurement with the Quant-It PicoGreen dsDNA assay. Similar. 15日开始对本试剂盒内标品（B组分）进行更新，由原来的小牛胸腺DNA升级为Lamda DNA，内部测试后者与Picogreen具更佳的结合性和荧光信号。后续统一按照新标品供货。Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). 2. A 96 plate well was filled with 100 µl of dye and 100 µl of DNA, a 384 well with 10 µl of each solution. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Rapidly detect as little as 0. Product Type. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of ssDNA and RNA with minimal effect on the quantitation results. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. 双链DNA（dsDNA）定量检测试剂盒 【产品升级声明】： 我司（懋康生物）从22. Catalog Number Specifications Unit Size Product Type Quantitation Range Packaging Type Price (EUR) Availability Quantity;DNA Quantification Using Quant-iT™ PicoGreen® The Quant-iT™ PicoGreen® assay was tested to overcome limitations of the UV absorbance assay at very low DNA concentrations. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. 5 mm in the Eppendorf BioSpectrometer fluorescence, PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. Quantitation Range. Final libraries were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a SpectraMax iD3 (Molecular Devices). Common contaminants such as salts, free nucleotides, solvents,Product Description. Sample fluorescence was. Quant-iT PicoGreen dsDNA Reagent. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. • Suitable for use in 96- and 384-well plate formats. The AccuBlue® Broad Range RNA Quantitation Kit is highly selective for RNA over dsDNA. Quant-iT PicoGreen dsDNA Reagent. An aliquot (1. Quant-IT dsDNA Assay Kit, high sensitivity Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target: dsDNA: dsDNA: dsDNA: Quantitation range: 0. 04 corresponds to 2 μ g/mL dsDNA solution. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. The tubes were. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. UV흡광 결과보다 민감도 우수. Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. 0 mL to 200 uL to fit a 96-well microplate format. no. • Specific for dsDNA in the presence of RNA. High linearity and sensitivity over a broad DNA range. a) 16. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. In the Quant-iT/PicoGreen assay, DNA and working solution were mixed 1:1. Price from $9. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). In case dsDNA standards are available only the quantification reagent can be used. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. Rapidly detect as little as 0. The Quant-iT™ dsDNA High-Sensitivity Kit is designed for assaying samples containing 0. The assay is a homogeneous, mix-and-read format where the fluorescence is proportional to analyte present. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Buy from Supplier. Bioz Stars score: 86/100, based on 1 PubMed citations. Quantitation using Quant-iT Assay Kits. Mix by gently vortexing for 5 seconds. 2023-11. 4). For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2. PicoGreen dsDNA quantitation reagents are. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 2 ng to 100 ng of DNA in the assay tube. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. low-range. Both fluorescent assays were performed with a Qubit fluorometer. quant it picogreen dsdna assay kit reagents. The libraries were sequenced using the Illumina HiSeq 2500. Thermo Fisher quant it picogreen dsdna quantification reagent kit Quant It Picogreen Dsdna Quantification Reagent Kit, supplied by Thermo Fisher, used in various techniques. PicoGreen assay. This sensitivity exceeds that achieved with the Hoechst 33258. For Research Use. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. 2 to 100 ng) Assay Kit were used with a Qubit 3. Picogreen assays are compatible with most microplate readers and fluorometers. Therefore, fluorescence spectroscopy using PicoGreen is more useful than UV spectroscopy for evaluating the template activity of DNA for PCR. to each well. Picogreen Reagent b. 使用 Quant-iT Picogreen dsDNA 定量试剂盒和 dsDNA 试剂在较宽的动态范围内实现精准的 dsDNA 浓度测定。. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Not for use in diagnostic procedures. Picogreen assays are compatible with most microplate readers and fluorometers. Bioz Stars score: 86/100, based on 1 PubMed citations. Picogreen assays are compatible with most microplate readers and fluorometers. Using this data as a starting point, the optimal conditions (e. 1 mL kit. Helixyte™ Green exhibits large fluorescence enhancement upon binding to dsDNA. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. BR reagent (Component A) 1. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Picogreen assays are compatible with most microplate readers and fluorometers. The dynamic range of this assay in microplate format, as stated in the product manual, is from 250 pg/mL to 1000 ng/mL with a single dye concentration. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant It. Thermo Fisher quant it picogreen dsdna quantitation assay kit. 95 mL TE for the . The Quant-iT™ DNA Assays are designed for high throughput (>20 samples) and for use in 96-well plate readers with no further adaptation. 2009;49(4):997–1002. • Suitable for use in 96- and 384-well plate formats. Quantitation Range. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. The Quant-iT™ dsDNA BR assay can be adapted for use in cuvettes or 384-well microplates. 10 x 100 µL. Picogreen assays are compatible with most microplate readers and fluorometers. Catalog Number. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Picogreen assays are compatible with most microplate readers and fluorometers. 使用 PicoGreen dsDNA 定量试剂盒和试剂时，即使在存在 ssDNA、RNA 和游离核苷酸的情况下，也可快速检测低至 0. PicoGreen dsDNA quantitation reagents are. Quant-iT ™ 1X dsDNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33232 Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267 Quant-iT ™ DNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33120 Quant-iT ™ DNA Assay Kit, Broad-Range 4 ng−1 μg 1,000 reactions Q33130 Quant-iT ™The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. PicoGreen Assay: CHO DNA Concentrate (10 µg/mL), 120 µL D553X Quant-iT PicoGreen dsDNA reagent D003 Solution, 1 x 1. 22. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT PicoGreen dsDNA Assay Kit. This protocol is a simplified and condensed. The micrococcal nuclease treatment used for fragmentation in some selected ChIP methods will generate. Module in combination with PicoGreen® dye allows the direct quantitation of dsDNA in as little as 100 µL total volume. Helixyte™ Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 3,201 3. PicoGreen dsDNA quantitation reagents are. No. Selection guide for the Qubit and Quant-iT Assay Kits—Table 8. Natural cures with herbs are. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. 1 mL kit. このアッセイは、従来のUV吸光度法に比べて感度が何倍も高いため、様々な濃度範囲に対して最適化することが可能です。. High Sensitivity and Broad Range dsDNA Quantitation Superior sensitivity and broad dynamic range A ccuBlue™ dsDNA quantitation kits offer ultra-sensitive and selective detection of dsDNA with minimal effects from common contaminants. • Suitable for use in 96- and 384-well plate formats. Rapidly detect as little as 0. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Product Type. The DNA amount in each library was quantified with the Quant-iT PicoGreen dsDNA Assay Kit and with the Agilent 2200 TapeStation. 2. 2. • Suitable for use in 96- and 384-well plate formats. (Left) The fluorescence signal from binding of the Quant-iT PicoGreen dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/ml calf thymus DNA. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 95 mL TE for the . Related applications: Nucleic Acid Quantitation. Picogreen assays are compatible with most microplate readers and fluorometers. The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). This protocol is a simplified and c. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. The assay is linear over three orders of magnitude and has little sequence. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitlimit consume only 2 picograms of dsDNA per measure-ment. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. Further to the Quant-iT dsAssay kit (PicoGreen®), the Qubit dsDNA HS Assay kit offers an additional option for quantify-ing dsDNA samples of low concentration on the BioSpec-trometer fluorescence with high specificity and sensitivity. Tweet. The PG reagent from the Quant-iT TM PicoGreen TM dsDNA Assay Kit (Invitrogen) was prepared immediately before use by making a 1:200 dilution of the PG in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. (2012). 2–100 ng of DNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Quant-iT™ PicoGreen dsDNA Assay Kit (Life Technologies; Cat. 8 Measurement of growth factor content in. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 005% Triton X-100, Tween-20, or Brij. 50 pg to 2 µg. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Detecte rápidamente tan solo 0,25 pg/ml de ADNbc, incluso en presencia de ADNmc. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 8 List of Tables Molecular Probes amine-reactive dyes—Table 1. 20 and 2. This was possible since, due to the low height of the light ray of only 8. 2. . Approximate fluorescence excitation/emission maxima: 510/527 nm (see Figure 1, page 2) Quant-iT ™ dsDNA Broad-Range Assay Kit Catalog no. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for. Rapidly detect as little as 0. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Buy from Supplier. Quant-iT PicoGreen dsDNA Reagent. Quant-iT dsDNA BR buffer (Component B) 250 ml c. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. The QFX is a powerful solution for all fluorometric applications where enhanced specificity or sensitivity are needed. Note: Allow the Quant-iT ™ RiboGreen RNA Reagent to warm to room temperature before. 2 - 100 ng/ml 480/520 NaCl, MgCl 2Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Quant It Picogreen Dsdna Quantitation Assay Kit, supplied by Thermo Fisher, used in various techniques. Rapidly detect as little as 0. 특징: Quanti-iT Picogreen을 이용하면 낮은 농도의 DNA 측정에 유리. 5. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. 3. Table 2 summarizes results of the. (D–G) TLR stimulation was measured using a HEK293-TLR reporter. Picogreen assays are compatible with most microplate readers and fluorometers. Q33130) Metabolic (cholesterol) • Amplex Red Cholesterol Assay Kit (Cat. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 2. 50 pg to 2 µg. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The fluorescence intensity of PicoGreen was measured to determine the. ” J Pharm Biomed Anal. The PicoGreen is sticking to the sides of the cuvette or assay plate. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitQuant-iT™ PicoGreen® dsDNA Reagent and Kits | 4 Experimental Protoco l DNA Standard Curve 1. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. 995 mL TE for the . This assay is designed for use with fluorescence microplate readers. The user guide provides detailed information on the product description, component concentration, storage, assay protocol, and safety data sheets of the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). No. using a PicoGreen assay by the company life technologies (Quant-iT™ PicoGreen dsDNA Reagent and Kits). 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. Quant-it Picogreen® dsDNA assay kit (ref P11496) was obtained from Thermofisher scientific. The normalized DNA was aliquoted into multiple tubes at 50µL volume. Rapidly detect as little as 0. The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. 8. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. Highly selective for dsDNA over ssDNA or RNA. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Free dye. dsDNA standards were incubated in cuvettes and measured using a Varian Cary Eclipse. Picogreen assays are compatible with most microplate readers and fluorometers. Rapidly detect as little as 0. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). The Quant-iTTM PicoGreenTM dsDNA Reagent enables researchers to quantitate as little as 250 pg/mL of dsDNA (50 pg dsDNA in a 200 μL assay volume) with a fluorescence. DNA samples with a concentration determined by 260 nm absorbance higher than 100 ng/mL were diluted. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesIn this research six cyanine fluorophores for the quantification of dsDNA in the pg-ng range, without amplification, are compared under exactly identical conditions: EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Table 2 summarizes results of the. Rapidly detect as little as 0. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. ” application on Lunatic and compared to Qubit and. Quant-iT dsDNA BR buffer (Component B) 250 ml c. 2. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Rapidly detect as little as 0. The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. Rapidly detect as little as 0. Sample cDNA concentration was measured using the Quant-iT PicoGreen dsDNA assay kit and black Nunc F96 MicroWell polystyrene plates (ThermoFisher Scientific) according to the manufacturer’s. The AccuBlue® Broad Range RNA Quantitation assay has a linear range of 5-1000 ng RNA in a fluorescent plate reader, a wider range than similar assays. 3. 2 to 100 ng) Assay Kit were used with a Qubit 3. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. Price from $9. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。.